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Raman & FLIM Imaging Tutorial: RaFLIM® using the RMS1000 Confocal Microscope Guide

Edinburgh Instruments
3.23K subscribers
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165 views 2 months ago
Do you need help performing Raman and FLIM images of the same part of your sample? This step-by-step tutorial covers everything you need to know. • FLIM setup: learn how to set laser and detector conditions in Ramacle® for fluorescence and phosphorescence lifetime, and then perform imaging with those conditions. • FLIM analysis: create images based on fluorescence intensity or lifetime, with up to four exponential component fits in Ramacle®. • Raman setup: learn how to set laser and scatter path conditions in Ramacle® for Raman imaging. • Raman analysis: create images based on various Raman spectral conditions on the same sample area or volume that you performed FLIM on! Thanks for watching, remember to like, comment and subscribe for content like this and more! Chapters: 0:00 – Intro 0:35 - Focusing our Sample 1:34 - PL Spectra Measurements 2:22 - Selecting Optimum Wavelength for FLIM Measurements 2:49 - Lifetime Measurements 3:38 - Lifetime Measurement Analysis 4:00 - Performing 2D Mapping - FLIM 6:50 - Performing 2D Mapping - Raman 8:12 - Outro Visit our Website: www.edinst.com ...more
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Raman & FLIM Imaging Tutorial: RaFLIM® using the RMS1000 Confocal Microscope Guide
11Likes
165Views
Mar 202025
Do you need help performing Raman and FLIM images of the same part of your sample? This step-by-step tutorial covers everything you need to know. • FLIM setup: learn how to set laser and detector conditions in Ramacle® for fluorescence and phosphorescence lifetime, and then perform imaging with those conditions. • FLIM analysis: create images based on fluorescence intensity or lifetime, with up to four exponential component fits in Ramacle®. • Raman setup: learn how to set laser and scatter path conditions in Ramacle® for Raman imaging. • Raman analysis: create images based on various Raman spectral conditions on the same sample area or volume that you performed FLIM on! Thanks for watching, remember to like, comment and subscribe for content like this and more! Chapters: 0:00 – Intro 0:35 - Focusing our Sample 1:34 - PL Spectra Measurements 2:22 - Selecting Optimum Wavelength for FLIM Measurements 2:49 - Lifetime Measurements 3:38 - Lifetime Measurement Analysis 4:00 - Performing 2D Mapping - FLIM 6:50 - Performing 2D Mapping - Raman 8:12 - Outro Visit our Website: www.edinst.com

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Edinburgh Instruments

3.23K subscribers