Cell Reports
Volume 33, Issue 6, 10 November 2020, 108356
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Prrx1 Fibroblasts Represent a Pro-fibrotic Lineage in the Mouse Ventral Dermis

https://doi.org/10.1016/j.celrep.2020.108356Get rights and content
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Highlights

  • Fibroblast subpopulations exist in the mouse dermis

  • Prrx1-expressing fibroblasts contribute to ventral skin scarring

  • Prrx1 defines fibroblasts in the mouse dermis with scar-forming potential

Summary

Fibroblast heterogeneity has been shown within the unwounded mouse dorsal dermis, with fibroblast subpopulations being identified according to anatomical location and embryonic lineage. Using lineage tracing, we demonstrate that paired related homeobox 1 (Prrx1)-expressing fibroblasts are responsible for acute and chronic fibroses in the ventral dermis. Single-cell transcriptomics further corroborated the inherent fibrotic characteristics of Prrx1 fibroblasts during wound repair. In summary, we identify and characterize a fibroblast subpopulation in the mouse ventral dermis with intrinsic scar-forming potential.

Keywords

fibrosis
paired related homeobox 1
Prrx-1
fibroblasts
wound healing
dermis
scarring
fibroblast heterogeneity
radiation-induced fibrosis
tumor stroma formation
single-cell RNA sequencing
RNA sequencing
RNA-seq
transcriptomics
cell barcoding

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These authors contributed equally

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