› 4q1q

X-ray diffraction
2.11Å resolution

Crystal structure of TibC-catalyzed hyper-glycosylated TibA55-350 fragment

Released:
DOI: 10.2210/pdb4q1q/pdb
PDB entry 4q1q coloured by chain, front view.
PDB entry 4q1q coloured by chain, side view.
PDB entry 4q1q coloured by chain, top view.
Source organism: Escherichia coli ETEC H10407
Primary publication:
An iron-containing dodecameric heptosyltransferase family modifies bacterial autotransporters in pathogenesis.
Lu Q, Yao Q, Xu Y, Li L, Li S, Liu Y, Gao W, Niu M, Sharon M, Ben-Nissan G, Zamyatina A, Liu X, Chen S, Shao F
Cell Host Microbe 16 351-63 (2014)
PMID: 25211077

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autotransporter adhesin/invasin TibA Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 30.17 KDa
Source organism: Escherichia coli ETEC H10407
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XD84 (Residues: 55-350; Coverage: 32%)
Gene names: ETEC_2141, tibA
Sequence domains: Adhesin of bacterial autotransporter system, probable stalk

Ligands and Environments

1 bound ligand:
Ligand 289 70 x 289
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 46.807Å b: 62.166Å c: 97.288Å
α: 90° β: 90.9° γ: 90°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli

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Citations

11 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
10 more

2 mentions without citation

A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family.
Yao et al. (2014)
1 more