› 4pz3

X-ray diffraction
1.08Å resolution

High-resolution crystal structure of the human CD44 hyaluronan binding domain complex with undefined peptides

Released:
DOI: 10.2210/pdb4pz3/pdb
PDB entry 4pz3 coloured by chain, front view.
PDB entry 4pz3 coloured by chain, side view.
PDB entry 4pz3 coloured by chain, top view.
Source organisms:
Primary publication:
High-resolution crystal structures of alternate forms of the human CD44 hyaluronan-binding domain reveal a site for protein interaction.
Liu LK, Finzel B
Acta Crystallogr F Struct Biol Commun 70 1155-61 (2014)
PMID: 25195884

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147594 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CD44 antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 16.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P16070 (Residues: 18-170; Coverage: 21%)
Gene names: CD44, LHR, MDU2, MDU3, MIC4
Sequence domains: Extracellular link domain
Undefined peptides modeled as AAAV Chain: C
Molecule details ›
Chain: C
Length: 4 amino acids
Theoretical weight: 330 Da
Source organism: Escherichia coli
Expression system: Escherichia coli BL21

Ligands and Environments

3 bound ligands:
Ligand PEG 1 x PEG
Ligand EDO 2 x EDO
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 72.498Å b: 60.288Å c: 75.134Å
α: 90° β: 113.4° γ: 90°
R-values:
R R work R free
0.167 0.166 0.177
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Revealing the Mechanisms of Protein Disorder and N-Glycosylation in CD44-Hyaluronan Binding Using Molecular Simulation.
Guvench O. (2015)
1 more

8 mentions without citation

High-resolution crystal structures of alternate forms of the human CD44 hyaluronan-binding domain reveal a site for protein interaction.
Liu et al. (2014)
7 more